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Cell Signaling Technology Inc
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Lifespan Biosciences
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Cell Signaling Technology Inc
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Proteintech
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Acetyl Stat3 Lys685 Polyclonal Antibody for Western Blot
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Rabbit polyclonal to Stat3 Acetyl Lys87 Conjugation note Unconjugated Application note WB ELISA Reactivity note Human Mouse Rat
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Image Search Results
Journal: Journal of Neuroinflammation
Article Title: CXCL13 contributes to chronic pain of a mouse model of CRPS-I via CXCR5-mediated NF-κB activation and pro-inflammatory cytokine production in spinal cord dorsal horn
doi: 10.1186/s12974-023-02778-x
Figure Lengend Snippet: STAT3 is activated and co-localized with CXCL13 in ipsilateral SCDH of CPIP mice. A Western blot showing expression of p-STAT3 and STAT3 in sham (left) and CPIP model (right) mice on Day 0, 7 and 14. Upper panels indicated representative blot images and lower panel indicate pooled data. B Western blot showing expression of acetylated-STAT3 (Ace-Stat3). n = 4–6 mice/group. C – E Double immunostaining of p-STAT3 with NeuN ( C ), GFAP ( D ) and Iba-1 ( E ) in ipsilateral SCDH of CPIP mice. White arrows indicate p-STAT3 and NeuN co-labeled cells. F Double immunostaining of p-STAT3 with CXCL13 in ipsilateral SCDH of CPIP mice. White arrows indicate p-STAT3 and CXCL13 co-labeled cells. Quantification of cellular distribution was shown on the right (pooled from 4 to 5 mice/group). * p < 0.05, ** p < 0.01 vs. Day 0. NS: no significance. Scale bar indicates 50 μm. One-way ANOVA followed by Tukey’s post hoc test was used for comparisons in A , B
Article Snippet: The 1st antibodies used are as follows: CXCL13 (#PA5-28827, 1:1000, rabbit polyclonal, Thermo Fisher), CXCR5 (#254415, 1:2000, rabbit monoclonal, Abcam), STAT3 (#9139, 1:1000, mouse monoclonal, CST), pSTAT3 (#9145, 1:2000, rabbit monoclonal, CST),
Techniques: Western Blot, Expressing, Double Immunostaining, Labeling
Journal: Journal of Neuroinflammation
Article Title: CXCL13 contributes to chronic pain of a mouse model of CRPS-I via CXCR5-mediated NF-κB activation and pro-inflammatory cytokine production in spinal cord dorsal horn
doi: 10.1186/s12974-023-02778-x
Figure Lengend Snippet: Activation of STAT3 is involved in CXCL13 upregulation in spinal cord dorsal horn and contributes to mechanical allodynia of CPIP mice. A Protocol illustrating specific time points for model establishment, intrathecal injections of NF-κB inhibitor PDTC/vehicle and behavioral test. B Western blot showing the expression of p-STAT3 and STAT3 in Sham + Veh, CPIP + Veh and CPIP + S3I-201 groups. Upper panels indicate representative blot images and lower panels indicate pooled data. C Western blot showing the expression of CXCL13 in all three groups. D 50% PWT changes in ipsilateral hindpaws after intrathecal treatment with S3I-201 or corresponding vehicle in CPIP and sham group of mice n = 5–6 mice/group. * p < 0.05, ** p < 0.01. One-way ANOVA followed by Tukey’s post hoc test was used for comparisons in B , C . Two-way ANOVA with repeated measures followed by Tukey’s post hoc test was used for comparisons in D
Article Snippet: The 1st antibodies used are as follows: CXCL13 (#PA5-28827, 1:1000, rabbit polyclonal, Thermo Fisher), CXCR5 (#254415, 1:2000, rabbit monoclonal, Abcam), STAT3 (#9139, 1:1000, mouse monoclonal, CST), pSTAT3 (#9145, 1:2000, rabbit monoclonal, CST),
Techniques: Activation Assay, Western Blot, Expressing
Journal: Brain Tumor Research and Treatment
Article Title: Anti-Migration and Anti-Invasion Effects of Curcumin via Suppression of Fascin Expression in Glioblastoma Cells
doi: 10.14791/btrt.2019.7.e28
Figure Lengend Snippet: Western blot analysis for quantification of fascin expression and sandwich ELISA for quantification of STAT3. A: Fascin expression temporarily decreased at 12 hours (18.4%, p =0.024), but gradually recovered after 12 hours. B: The change of phosphorylated STAT3 level also reflected temporarily decreased patterns of fascin expression at 12 hours (19.7%, p =0.010). Whereas concentrations of total STAT3 according to a time sequence were similar. Data were presented as the mean±SD. * p <0.05 compared to the control group. STAT3, signal transducer and activator of transcription 3.
Article Snippet: The concentrations of total or phosphorylated STAT3 in the samples were determined using sandwich-ELISA kits [
Techniques: Western Blot, Expressing, Sandwich ELISA, Sequencing